Desert Southwest Genomics Center

Overview of Services

The Desert Southwest Genomics Center (DSGC) is a sequencing facility at Arizona State University offering scientists and researchers within ASU and worldwide the opportunity to perform a full range of genomic applications, such as Whole Genome Sequencing (WGS), RNA sequencing (including options for poly(A) selection, ribosomal depletion, and degraded/FFPE RNA), library preparation of ChIP samples, de novo sequencing, metagenomics sequencing, single-cell sequencing and traditional Sanger sequencing.

All next generation sequencing applications are performed on the Illumina platform with the MiSeq, NextSeq, and NovaSeq X instruments, using the principle of “sequencing by synthesis," to generate millions of sequence fragments that can then be assembled into a genome, transcriptome, exome, or amplicon library as relevant. The two sequencers in the core provide data output ranging from 1 million reads to 3.25 billion reads in varying read lengths, enabling the core to sequence projects ranging from proof-of-concept to genome assembly. Learn more about the Illumina platform.

The DSGC has expanded services to include long-read sequencing utilizing Pacific Biosciences Revio. This allows researchers to conduct Whole Genome Sequencing, RNA, microbiome, metagenomes, and epigenetics. Learn more about the Pacific Biosciences platform

Recently, the facility acquired an upgraded 10X Genomics instrument for sample partitioning and molecular barcoding prior to sequencing. The facility is pleased to be able to offer single-cell sequencing pipelines to the ASU community through this platform. Learn more about the 10X Genomics platform.

For metagenomics sequencing, 16S, 18S, or ITS regions can be isolated and sequenced as relevant and are sequenced on the NextSeq2000 instrument, allowing researchers to characterize and study microbial communities from various environments such as soils, freshwater, seawater, wastewater, and host-microbe associations. More information about the primer sets used for targeted metagenomics can be found on our Information and Resources tab.

The DSGC also maintains a close partnership with the ASU Bioinformatics Core, providing researchers with access to a informatics team experienced with NGS sample processing for downstream informatics needs. Learn more about the Bioinformatics Facility.

The core has also made several instruments available for researchers to use independently. Currently, an Agilent Tapestation, a Quantstudio 7, a Covaris M220, Qubit Flex, and a Qubit4 are open for scheduled use following training by a facility staff member. Please visit our Equipment tab for more details about our instruments or contact us to schedule training.

In addition, the expertise of facility staff is available for researchers at any point in the experimental process. Please contact the core to set up a consultation for any questions!

Leadership

Joy Blain, Ph.D

Desert Southwest Genomics Center Manager

joy.blain@asu.edu

(480) 965-4998

 

Kristina Buss

Bioinformatics Core Manager

kristina.buss@asu.edu

 

Anna Engelbrektson

NGS Principal Research Specialist

aengelb3@asu.edu

  

Candyce McDaniel

Research Specialist

ccochra@asu.edu

 

Lei Zuo

Bioinformatic Scientist

lei.zuo@asu.edu

 

Location and Hours of Operation

Hours Location

Monday - Friday     

8 am to 4:30 pm

Walton Center for Planetary Health

777 E. University Dr. Room 530

Tempe, AZ 85287-7604

For information on For-Profit rates, contact the core manager.

 

Contacts

Name Role Phone Email Location
Desert Southwest Genomics Center

 
(480) 965-6795
 
genomicshelp@asu.edu
 

 
Bioinformatics

 

 
asubioinformatics@asu.edu
 

 

Services List


Search available services: View: by category alphabetically
NGS Bioinformatics Analysis (14)
NGS Run Modules - MiSeq (6)
NGS Sample Prep - DNA (3)
NGS Sample Prep - Microbiome (5)
NGS Sample Prep - RNA (7)
Nucleic Acid Extraction (2)
Quality Control (6)
Sanger Sequencing (5)
Training (1)
Long-Read Sequencing Facility (10)
NGS TGen Sequencing (4)
NextSeq (7)

Available Equipment and Resources

Name Price
FLX800TBI Fluorescent Plate Reader  
FLX800TBI Fluorescent Plate Reader Sun-Sat 12:00 AM - 12:00 AM
ASU Standard $12.00 /hr
Academic/Non-Profit $15.00 /hr
QuantStudio 7 Flex  
Trained Sun-Sat 12:00 AM - 12:00 AM
ASU Standard $18.00 /hr
Academic/Non-Profit $20.00 /hr