Genomics Core

Overview of Services

The KED Genomics Core, a sequencing facility at Arizona State University, has been in operation since 2009, offering scientists and researchers within ASU and worldwide the opportunity to perform a full range of genomic applications, such as Whole Genome Sequencing (WGS), RNA sequencing (including options for poly(A) selection, ribosomal depletion, and degraded/FFPE RNA),library preparation of ChIP samples, de novo sequencing, metagenomics sequencing, single-cell sequencing, and traditional Sanger sequencing.

All next generation sequencing applications are performed on the Illumina platform with the MiSeq and NextSeq instruments, using the principle of “sequencing by synthesis” to generate millions of sequence fragments that can then be assembled into a genome, transcriptome, exome, or amplicon library as relevant. The two sequencers in the core provide data output ranging from 1 million reads to 400 million reads in varying read lengths, enabling the core to sequence projects ranging from proof-of-concept to genome assembly. Learn more about the Illumina platform.

Recently, the core acquired a 10X Genomics instrument for sample partitioning and molecular barcoding prior to sequencing, and is pleased to be able to offer single-cell sequencing pipelines to the ASU community through this platform. Learn more about the 10X Genomics platform.

For metagenomics sequencing, 16S, 18S, or ITS regions can be isolated and sequenced as relevant and are sequenced on the MiSeq instrument, allowing researchers to characterize and study microbial communities from various environments such as soils, freshwater, seawater, waste water, and host-microbe associations. More information about the primer sets used for targeted metagenomics can be found on our Information and Resources tab.

The Genomics Core also maintains a close partnership with the ASU Bioinformatics Core, providing researchers with access to a Ph.D level informatics team experienced with NGS sample processing for downstream informatics needs. Learn more about the Bioinformatics Core.

The core has also made several instruments available for researchers to use independently. Currently, an Agilent Tapestation, a Quantstudio 5, a Covaris M220, and a Qubit4 are open for scheduled use following training by a member of the core’s staff. Please visit our Equipment tab for more details about our instruments, or contact us to schedule training.

In addition, the expertise of the Genomics Core staff is available for researchers at any point in the experimental process. Please contact the core to set up a consultation for any questions!

Leadership

Joy Blain, Ph.D

Genomics Core Manager

joy.blain@asu.edu

(480) 965-4998

 

Shanshan Yang, Ph.D

Bioinformatics Core Manager

Shanshan.Yang.1@asu.edu

 

Juan Maldonado, Ph.D

Microbiome, Research & Developement

juan.maldonadoortiz@asu.edu

 

Kristina Buss 

NGS Research Specialist 

kristina.buss@asu.edu 

 

Nick Mellor

Research Technician

Nicholas.Mellor@asu.edu

 

Dee Plueard

Research Technician

dee.plueard@asu.edu

 

Brian Hanratty

Bioinformatics Research Specialist

Brian.Hanratty@asu.edu

 

Location and Hours of Operation

Hours Location

Monday - Friday     

8 am to 4:30 pm

Biodesign Institute

1001 S. McAllister Ave

Tempe, AZ  85287

 

For information on For-Profit rates, contact the core manager.

Contacts

Name Role Phone Email Location
Genomics

 
(480) 965-4998
 
genomicshelp@asu.edu
 

 
Bioinformatics

 

 
asubioinformaics@asu.edu
 

 

Services List


Search available services: View: by category alphabetically
NGS Bioinformatics Analysis (19)
NGS Run Modules - MiSeq (6)
NGS Run Modules - NextSeq (5)
NGS Run Modules - Sequencing Reads (1)
NGS Sample Prep - DNA (4)
NGS Sample Prep - Microbiome (5)
NGS Sample Prep - RNA (9)
Nucleic Acid Extraction (2)
Quality Control (11)
Sanger Sequencing (6)
Training (1)

Available Equipment and Resources

Name Price
Covaris  
Unassisted Use Prime Time Mon-Fri 08:00 AM - 04:00 PM
ASU Standard $8.00 /hr
Academic/Non-Profit $8.00 /hr
Unassisted Use Off Prime Time Sun-Sat 12:00 AM - 12:00 AM
ASU Standard $8.00 /hr
Academic/Non-Profit $8.00 /hr
TapeStation - Agilent  
Standard Availability Mon-Fri 08:00 AM - 05:00 PM
ASU Standard $8.00 /hr
Academic/Non-Profit $8.00 /hr
Extended Availability Mon-Fri 05:00 PM - 10:00 PM
Academic/Non-Profit $8.00 /hr
ASU Standard $8.00 /hr