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SOLS Shared Resource Facility
Real time PCR in the SOLS SRF is run on an ABI7900HT thermocycler using a 384 well format. Samples are usually set up in respective laboratories. For training on the software and help in experimental setup, please reach out to regenmed@asu.edu. Primers and probes can be designed using ABI Primer Express software.
The Biotek HT1 Plate Reader scans multiple plate types in Absorbance, Fluorescence or Luminescence modes. Training is provided on the software and plate setup.
Room Access
To obtain ISAAC access to LSE S05 - Submit your request using this SOLS Shared Core Access Form. LSE S-05 requires key access. Users must request SAM box access through the SOLS Facilities office. Authorized users will open the SAM box on the 2nd floor (near the LSC/LSE connecting bridge - It looks like a breaker switch box) with their ASU card and will have access to check out the S-05 key. Should you forget to return a key, the SAM Box will alert the user the same day via email and informs them the key must be returned. If the user does not respond or return the key, all access privileges will be revoked.
Location and hours of operation
| Mailing Address | Shipping Address |
|
SOLS Shared Resource Lab School of Life Sciences PO Box 874501 ASU, Tempe AZ 85282-4501 |
SOLS Shared Resource Lab School of Life Sciences 427 E. Tyler Mall ASU, Tempe AZ 85287-4501 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Genomics Core |
Fragment Analysis
|
(480) 965-4998
|
genomicshelp@asu.edu
|
|
| Regenerative Medicine Core |
Instrument Training
|
regenmed@asu.edu
|
| Services List |
| ► Sanger Sequencing (4) | |||
| Name | Description | Price | |
|---|---|---|---|
| Sanger Sequencing - Individual Samples: up to 47 |
Individual samples up to 47. |
ASU Standard
$3.85
Sample
Academic/Non-Profit $4.24 Sample |
|
| Sanger Sequencing Plates: Minimum of 48 Samples |
Sanger sequencing plates per sample with a minimum of 48 samples required. |
ASU Standard
$3.12
Sample
Academic/Non-Profit $3.85 Sample |
|
| Sequencing Clean and Run - Individual Samples: up to 47 |
Individual samples up to 47. |
ASU Standard
$0.73
Sample
Academic/Non-Profit $0.88 Sample |
|
| Sequencing Clean and Run Plates: Minimum of 48 Samples |
Minimum of 48 samples. |
ASU Standard
$0.59
Sample
Academic/Non-Profit $0.71 Sample |
|
| ► PCR Purification (1) | |||
| Name | Description | Price | |
| PCR Purification |
Purify samples for sequencing. |
ASU Standard
$0.53
Sample
Academic/Non-Profit $0.85 Sample |
|
| ► Fragment Analysis (2) | |||
| Name | Description | Price | |
| Fragment Runs - Clean and Run: Size Standard Provided by Customer |
Clean and run, size standard provided by customer. |
ASU Standard
$0.59
Sample
Academic/Non-Profit $0.70 Sample |
|
| Fragment Runs - Clean and Run: Size Standard Provided by DNA Lab |
Clean and run, size standard provided by DNAlab. |
ASU Standard
$0.75
Sample
Academic/Non-Profit $0.85 Sample |
|
| ► Plasmid Preps (1) | |||
| Name | Description | Price | |
| Plasmid Preps |
Isolation of Plamid DNA from E. coli for Sequencing. |
ASU Standard
$0.82
Sample
Academic/Non-Profit $1.25 Sample |
|
| ► Instrument Use (3) | |||
| Name | Description | Price | |
| ABI 7900 HT Real Time PCR - Per Run w/ Customer-Owned Plate |
ASU Standard
$12.00
each
Academic/Non-Profit $17.00 each |
||
| ABI 7900 HT Real Time PCR - Per Run w/ DNA Lab Plate |
ASU Standard
$17.00
each
Academic/Non-Profit $22.00 each |
||
| Biotek HT1 Plate Reader - Per Session |
|
ASU Standard
$4.00
block
Academic/Non-Profit $5.00 block |
|
| Name | Price | ||
|---|---|---|---|
| ABI 7900 HT Real time PCR | |||
| Default additional charges | |||
| ABI 7900 HT Real Time PCR - Per Run w/ Customer-Owned Plate |
ASU Standard
$12.00
each
Academic/Non-Profit $17.00 each |
||
| ABI 7900 HT Real Time PCR - Per Run w/ DNA Lab Plate |
ASU Standard
$17.00
each
Academic/Non-Profit $22.00 each |
||
| Biotek HT1 Plate Reader | |||
| Default additional charges | |||
| Biotek HT1 Plate Reader - Per Session |
ASU Standard
$4.00
block
Academic/Non-Profit $5.00 block |
||
| Nanodrop ND1000 | |||
| NanoVue Spectrophotometer | |||