ALERT

ATTENTION ⚠️

On Saturday July 20th, 2024 at 4:30AM (UTC), maintenance has been scheduled for iLab Primary U.S.. Maintenance is planned for 4 hours and during this time, there is a server downtime of 2 hours and iLab site will be unavailable. Details and updates can be found on the Agilent Status Page

For additional information, please contact the Agilent iLab Support by clicking on 'Help' at the top of your account page or by emailing ilab-support@agilent.com.

DNA Synthesis Lab (DNASL)

Overview of Services

The DNA Synthesis Lab offers ASU researchers the ability to synthesize many different types of DNA or RNA products:

  • Linear DNA Fragments: 32 different fragments can be synthesized simultaneously of lengths 300-1800bp. All DNA fragments produced are processed with proprietary error correction, resulting in the lowest error rates available at 1:10,000-30,000.
  • Long Fragment DNA Synthesis: 300-7200bp fragments can be produced; these fragments are also compatible with our automated Gibson Assembly protocol.
  • Gibson Assembly: DNA fragments produced can be taken through our automated Gibson Assembly protocol. The protocol supports up to 4 vectors at a time with either 1 or 2 fragment cloning.
  • Transfection grade DNA: 1-24 DNA fragments of size 300-1800bp Gibson Assembled and subjected to RapidAMP amplification will yield more than 9 micrograms of transfection grade DNA.
  • mRNA Synthesis: With 1-16 samples at a time of size 300-1800bp will yield 1-10 micrograms of mRNA. Includes poly-A tails.
  • Barcoding and Protein Scanning Libraries: Essentially no upper-limit as to the amount of variation found in the sequences through the use of IUPAC nucleotide coding. Minimum size of fragments is 300bp. 

The synthesis lab utilizes a Codex DNA BioXP 3200 to offer all of these different products.

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IMPORTANT INFORMATION

Researchers will first need to create and place their order with Codex DNA before submitting to the DNA Synthesis Lab. 

Orders from Codex should be shipped to: 

1001 S McAllister Ave

Biodesign Institute BLDG A (BDA)

Tempe, AZ 85287

ATTN: Stikeleather BDC436

Completed orders, or Gibson-ready vectors can be picked-up/dropped-off  from:

Biodesign C430 Linear Equipment Freezer

Leadership

Ryan Stikeleather

Location and hours of operation

Hours Location

Monday - Friday; 9am to 5pm

Biodesign C436

 

Contacts

Name Role Phone Email Location
Ryan Stikeleather

 

 
Ryan.Stikeleather@asu.edu