DNA Synthesis Lab (DNASL)

Overview of Services

The DNA Synthesis Lab offers ASU researchers the ability to synthesize many different types of DNA or RNA products:

• Linear DNA Fragments: 32 different fragments can be synthesized simultaneously of lengths 300-1800bp. All DNA fragments produced are processed with proprietary error correction, resulting in the lowest error rates available at 1:10,000-30,000.

• Long Fragment DNA Synthesis: 300-7200bp fragments can be produced; these fragments are also compatible with our automated Gibson Assembly protocol.
  

• Gibson Assembly: DNA fragments produced can be taken through our automated Gibson Assembly protocol. The protocol supports up to 4 vectors at a time with either 1 or 2 fragment cloning.
  

• Transfection grade DNA: 1-24 DNA fragments of size 300-1800bp Gibson Assembled and subjected to RapidAMP amplification will yield more than 9 micrograms of transfection grade DNA.

• mRNA Synthesis: With 1-16 samples at a time of size 300-1800bp will yield 1-10 micrograms of mRNA. Includes poly-A tails.

• Barcoding and Protein Scanning Libraries: Essentially no upper-limit as to the amount of variation found in the sequences through the use of IUPAC nucleotide coding. Minimum size of fragments is 300bp. 

The synthesis lab utilizes a Codex DNA BioXP 3200 to offer all of these different products.

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IMPORTANT INFORMATION

Researchers will first need to create and place their order with Codex DNA before submitting to the DNA Synthesis Lab. 

Orders from Codex should be shipped to: 

1001 S McAllister Ave

Biodesign Institute BLDG A (BDA)

Tempe, AZ 85287

ATTN: Stikeleather BDC436

Completed orders, or Gibson-ready vectors can be picked-up/dropped-off  from:

Biodesign C430 Linear Equipment Freezer

Leadership

Ryan Stikeleather

Location and hours of operation

Contacts